The following information is vitally important to understanding just how dangerous genetically modified organisms (GMOs) are, not only to the environment, but to the health of everyone on the planet.
One of the nation’s senior scientists alerted the federal government to a newly discovered organism that may have the potential to cause infertility and spontaneous abortion in farm animals, raising significant concerns about human health. Dr. Don Huber, professor emeritus at Purdue University, believes the appearance and prevalence of the unnamed organism may be related to the nation’s over reliance on the weed killer known as Roundup and/or to something about the genetically engineered Roundup-Ready crops. In a letter to Secretary of Agriculture Tom Vilsack, the professor called on the federal government to immediately stop deregulation of roundup ready crops, particularly roundup ready alfalfa.
It is the genetic engineering process itself that is a clear and present danger to us all. The following GMO primer explains the process.
Dr. Mae-Wan Ho has been warning us for years that new forms of bacteria and viruses that have never before been seen will come about due to horizontal transfer inherent in the genetic engineering process. Glyphosate also causes severe problems, however, it is horizontal gene transfer that causes new “unnamed organisms.”
Barbara H. Peterson
Scientists Discover New Route for GM-gene ‘Escape’
Reprinted with permission
Source: ISIS (The Institute of Science in Society)
Genetically modified genes can jump species via wounds, yes horizontal gene transfer happens, and at high frequencies; it is the greatest, most underestimated hazard from GMOs released into the environment. Dr. Mae-Wan Ho
Please circulate widely and forward to your elected representatives
Gene “escape” a misnomer for horizontal gene transfer
Scientists at Bristol University in the UK announced the discovery of  “a previously unknown route” whereby “GM genes may escape into the natural environment.” “Escape” is a misnomer. There is no need for the GM (genetically modified) genes to “escape”, when genetically modified organisms (GMOs) have been released in great abundance and with gay abandon into the environment over the past 17 years. At issue is how fast and how widely the GM genes can spread, and what dire consequences could arise.
The “escape” referred to is horizontal gene transfer – the spread of GM genes by infection and multiplication (literally like a virus) regardless of species barriers; hence the rate of spread is much more rapid, and the extent virtually unlimited. New combinations of genetic material are created at unprecedented speed; affecting species the most that reproduce the fastest, i.e., bacteria and viruses that cause diseases. Horizontal gene transfer and recombination is indeed a main route for generating new strains of bacteria and viruses that cause diseases. Genetic modification and release of GMOs into the environment is nothing if not greatly facilitated horizontal gene transfer and recombination. It has created highways for gene trafficking in place of narrow by-ways and occasional footpaths that previously existed.
Some of us have long considered horizontal gene transfer to be the most serious hidden and underestimated hazard of genetic engineering, and have alerted regulators accordingly, time and again, since GMOs were first released (see for example [3, 4] (Gene Technology and Gene Ecology of Infectious Diseases, ISIS scientific publication; Genetic Engineering Dream or Nightmare, ISIS publication). The recent “emergency” warning sent by a senior US Department of Agriculture scientist to US Secretary of Agriculture on a suspected pathogen “new to science” associated with GM crops may prove to be a case in point  (Emergency! Pathogen New to Science Found in Roundup Ready GM Crops? SiS 50).
Plant wounds hotspots for gene trafficking
The researchers at Bristol University showed that plant wounds, that could be created by insect bites, abrasion and other mechanical damage, are hotspots for gene trafficking due to the wound hormones produced by the plant. Under such circumstances, the soil bacterium Agrobacterium tumefaciens, which causes crown gall disease in plants, could enlarge its host range to infect fungi, and insert foreign genes into the fungi’s genome . This has large implications on the safety of GMOs already widely released into the environment.
A. tumefaciens is probably unique among natural plant pathogens in carrying out trans-Kingdom horizontal gene transfer during an infection, and it is this ability that has been widely exploited for creating GM crops, grown on an estimated 134 million hectares worldwide in 2009, and “jumped’ another 10 percent in 2010, according to industry-funded International Service for the Acquisition of Agri-biotech Applications (ISAAA) .
Research commissioned by the UK Department of the Environment, Food and Rural Affairs (DEFRA) in the 1990s had already revealed that it is very difficult, if not impossible to get rid of the Agrobacterium vector used in creating the transgenic plant , and the bacterium is likely to remain dormant even after the transgenic plants are transplanted into the soil. Hence, it is expected to facilitate horizontal gene transfer, in the first instance, to wild-type Agrobacterium in the soil, and further afield.
Disease-causing strains of A. tumefaciens have an extrachromosomal Ti (tumour-inducing) plasmid that enables the horizontal transfer of a segment of the Ti plasmid, the T-DNA, into the plant cell genome when the bacterium’s virulence (disease causing) system is activated by hormones produced by the wounded plant. This feature is exploited in creating genetically modified organisms (GMOs), by disarming the bacterium, and incorporating the virulence genes in a ‘binary’ vector that has to be used in conjunction with the disarmed Agrobacterium strain.
In the 1990s, it was shown that the range of organisms transformed by Agrobacterium could be extended if the wound hormone acetosyringone was used to induce the virulence system.
The researchers at Bristol University reasoned that as A. tumefaciens is a soil-dwelling pathogen that often infects plants through wounds, it is conceivable that the bacterium could encounter numerous species of microorganisms, including pathogenic fungi that the same method to gain entry into the plant. The wound sites are likely to be exuding wound hormones such as acetosyringone, so the bacteria are primed for T-DNA transfer.
Experiments confirmed their suspicion in full
They carried out their investigation using the wilt-causing fungus Verticillium albo-atrum, a strong candidate for encounters with Agrobacterium in the plant, as it has a similar wide host range in plants, infecting both root and crown. Previous lab experiments have shown that V. albo-atrum cannot be transformed by Agrobacterium in the absence of acetosyringone. So, if it is presented with Agrobacterium on plant tissue, and transformation does occur, it must be the plant that supplies the wound hormone.
Peeled and sliced potato tubers and carrots, leave- and stem-sections from tobacco plants were used as the plant tissues for testing. After sterilization, they were inoculated with both A. tumefaciens and V. albo-atrum and left at room temperature in a covered agar dish for a minimum of 8 days and a maximum of 42 days.
Successful transformants of V. albo-atrum were obtained from every kind of plant tissue. 2 out of 17 potato slices, 1 out of 15 carrot slices; 14 out of 42 dishes each with 3-5 leaf pieces, and 10 out of 31 stem sections (without agar plate, so as to be as close to the natural condition as possible). These transformants were confirmed with molecular genetic analyses.
Implications on risk assessments of GMOs still understated
The researchers concluded : “This work therefore raises interesting questions about whether the host range of A. tumefaciens in nature is greater than just plants. It is possible that evidence of such events could be looked for retrospectively in the increasing number of genome sequences becoming available….
“In addition, the result may well have implications for the risk assessment of GM plants generated via Agrobacterium-mediated transformation, as Agrobacterium can survive within plant tissue through transformation and tissue culture and can therefore be found within regenerated transgenic plants…”
This is an understatement of a serious risk that has been known almost since the first release of Agrobacterium-transformed GMOs into the environment.
The risks are far greater than admitted
We have repeatedly drawn attention to the possibility of facilitated horizontal gene transfer from GMOs created with Agrobacterium vector, which is even stronger than originally envisaged due to other discoveries made since then. I reproduce what we wrote in 2008  (Horizontal Gene Transfer from GMOs Does Happen, SiS 38), which repeats an earlier account  (Living with the Fluid Genome , ISIS publication) (see Box).
Agrobacterium vector a vehicle for facilitated horizontal gene transfer [8, 9]
“We have ..provided evidence strongly suggesting that the most common method of creating transgenic plants may also serve as a ready route for horizontal gene transfer [9, 10].
“Agrobacterium tumefaciens, the soil bacterium that causes crown gall disease, has been developed as a major gene transfer vector for making transgenic plants. Foreign genes are typically spliced into the T-DNA – part of a plasmid of A. tumefaciens called Ti (tumour-inducing) – which ends up integrated into the genome of the plant cell that subsequently develops into a tumour.
“But further investigations revealed that the process whereby Agrobacterium injects T-DNA into plant cells strongly resembles conjugation, the mating process between bacterial cells.
Conjugation, mediated by certain bacterial plasmids requires a sequence called the origin of transfer (oriT) on the DNA that’s transferred. All the other functions can be supplied from unlinked sources, referred to as ‘trans-acting functions’ (or tra). Thus, ‘disabled’ plasmids, with no trans-acting functions, can nevertheless be transferred by ‘helper’ plasmids that carry genes coding for the trans-acting functions. And that’s the basis of a complicated vector system devised, involving Agrobacterium T-DNA, which has been used for creating numerous transgenic plants.
“It soon transpired that the left and right borders of the T-DNA are similar to oriT, and can be replaced by it. Furthermore, the disarmed T-DNA, lacking the trans-acting functions (virulence genes that contribute to disease), can be helped by similar genes belonging to many other pathogenic bacteria. It seems that the trans-kingdom gene transfer of Agrobacterium and the conjugative systems of bacteria are both involved in transporting macromolecules, not just DNA but also protein.
“That means transgenic plants created by the T-DNA vector system have a ready route for horizontal gene escape, via Agrobacterium, helped by the ordinary conjugative mechanisms of many other bacteria that cause diseases, which are present in the environment.
“In fact, the possibility that Agrobacterium can serve as a vehicle for horizontal gene escape was first raised in 1997 in a study sponsored by the UK Government [7, 12], which found it extremely difficult to get rid of the Agrobacterium in the vector system after transformation. Treatment with an armoury of antibiotics and repeated subculture of the transgenic plants over 13 months failed to get rid of the bacterium. Furthermore, 12.5 percent of the Agrobacterium remaining still contained the binary vector (T-DNA and helper plasmid), and were hence fully capable of transforming other plants.
“Agrobacterium not only transfers genes into plant cells; there is possibility for retrotransfer of DNA from the plant cell to Agrobacterium . High rates of gene transfer are associated with the plant root system and the germinating seed, where conjugation is most likely . There, Agrobacterium could multiply and transfer transgenic DNA to other bacteria, as well as to the next crop to be planted. These possibilities have yet to be investigated empirically.
“Finally, Agrobacterium attaches to and genetically transforms several human cell lines [15, 16] (Common plant vector injects genes into human cells ISIS News 11/12). In stably transformed HeLa cells (a human cell line derived originally from a cancer patient), the integration of T-DNA occurred at the right border, exactly as would happen when it is transferred into a plant cell genome. This suggests that Agrobacterium transforms human cells by a mechanism similar to that which it uses for transforming plants cells.
“The possibility that Agrobacterium is a vehicle for horizontal transfer of transgenic DNA remains unresolved to this day.”
Agrobacterium transfers genes into human cells
It is also worth reiterating our comment on the scientific paper  documenting that Agrobacterium can transfer genes into human cells .
“The paper shows that human cancer cells along with neurons and kidney cells were transformed with the Agrobacterium T-DNA. Such observations should raise alarm for those who use Agrobacterium in the laboratory.
“The integrated T-DNA will almost certainly act as a mutagen as it integrates into human chromosomes. Cancer can be triggered by activation of oncogenes (ie, cancer genes) or inactivation of cancer-suppressing genes. Furthermore, the sequences carried within the T-DNA in the transforming bacterium can be expressed in the transformed cells (the viral promoter CaMV has been found to be active in HeLa cells ) ….
“It is clear that little has been done to prevent environmental escape of the transforming bacteria or to quantify such releases. In conclusion, a study of cancer incidence among those exposed to Agrobacterium tumefaciens in the laboratory and in the field is needed. It would be worthwhile to screen workers for T-DNA sequences.”
The discovery by the Bristol University researchers barely scratches the surface of the hidden hazards of GMOs from horizontal gene transfer. It is high time for a global ban to be imposed on further environmental releases of GMOs, and all those responsible for releasing them should be brought to book.
1. “Hazards of GMOS: Agrobacterium mediated transformation”http://www.bristol.ac.uk/news/2010/7279.html
2. Knight CJ, Bailey AM, Foster GD. Investigating Agrobacterium-mediated transformation of Verticillium albo-atrum on plant surfaces. PLOS ONE 2010, 5(10): e13684. Doi:10.1371/journal.pone.0013684
3. Ho MW. Gene technology and gene ecology of infectious diseases. Microbial Ecology in Health and Disease 1998, 10, 33-59.
4. Ho MW. Genetic Engineering Dream of Nightmare? The Brave New World of Bad Science and Big Business, Third World Network, Gateway Books, MacMillan, Continuum, Penang, Malaysia, Bath, UK, Dublin, Ireland, New York, USA, 1998, 1999, 2007 (reprint with extended Introduction). http://www.i-sis.org.uk/genet.php
5. Ho MW. Emergency! Pathogen new to science found in Roundup Ready GM crops? Science in Society 50 (to appear).
6. Global Status of Commercialized Biotech/GM Crops, 2009, ISAAA, http://www.isaaa.org/
7. Mc Nicol MJ, Lyon GD, Chen MY, Barrett C and Cobb E. Scottish Crop Research Institute. Contract No RG 0202.The Possibility of Agrobacterium as a Vehicle for Gene Escape. MAFF. R&D and Surveillance Report: 395.
8. Ho MW and Cummins J. Horizontal gene transfer from GMOs does happen. Science in Society 38, 22-24, 2008
9. Ho MW. Living with the Fluid Genome, ISIS/TWN, London/Penanag, 2003. http://www.i-sis.org.uk/fluidGenome.php
10. Ferguson G and Heinemann J. Recent history of trans-kingdom conjugation . In Horizontal Gene Transfer 2nd ed., Syvanen M and Kado CI. (eds.) Academic Press, San Diego, 2002.
11. Ho MW. Horizontal gene transfer, book review. Heredity 2003, 90, 6-7.
12. Barrett C, Cobb E, MacNicol R and Lyon G. A risk assessment study of plant genetic transformation using Agrobacterium and implication for analysis of transgenic plants.Plant Cell Tissue and Organ Culture 1997, 19,135-144.
13. Kado C. in Horizontal Gene Transfer 2nd ed., Syvanen M and Kado CI. (eds.) Academic Press, San Diego, 2002.
14. Sengelov G, Kristensen KJ, Sorensen AH, Kroer N, and Sorensen SJ. Effect of genomic location on horizontal transfer of a recombinant gene cassette between Pseudomonas strains in the rhizosphere and spermosphere of barley seedlings. Current Microbiology 2001, 42, 160-7.
15. Kunik T, Tzfira T, Kapulnik Y, Gafni Y, Dingwall C, and Citovsky V. Genetic transformation of HeLa cells by Agrobacterium. PNAS USA, 2001, 98, 1871-87.
16. Cummins J. “Common plant vector injects genes into human cells. ISIS News 2002, 11/12, p. 10.
17. Ho MW, Ryan A and Cummins J. CaMV 35S promoter fragmentation hotspot confirmed and it is active in animals. Microbial Ecology in Health and Disease, 2000, 12, 189.
© 2011 Dr. Mae-Wan Ho